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KMID : 1024420150190010076
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Food Engineering Progress
2015 Volume.19 No. 1 p.76 ~ p.81
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In Vitro Antioxidant Activity of Ethanol Extract from Boehmeria nivea L. Leaves
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Kim Chi-Kwang
In Man-Jin
Kim Dong-Chung
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Abstract
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To investigate the antioxidative potential of ramie (Boehmeria nivea L.) leaves, alcoholic extracts with different ethanol concentration were prepared. The extract obtained by using 70% ethanol possessed the highest total phenolic content. The extraction yield and total phenolic content of the ethanolic extract were 15.46% and 105.0 μg chlorogenic acid equivalent (CAE)/mg, respectively. Therefore, the antioxidant activities of the extract were evaluated as 2,2diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, reducing power and nitrite scavenging ability. EC 50 value for radical scavenging and nitrite scavenging activities, which is the effective concentration at which 50% of DPPH radicals and nitrites are scavenged, were 34.72 μg/mL and 52.99 μg/mL, respectively. EC 50 value for reducing power, which is the effective concentration at which the absorbance is 0.5, is 44.39 μg/mL. All antioxidant activities increased as extract concentration increased. These results imply that the ethanolic extract of ramie leaves has the potential to be utilized as an effective antioxidant source.
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KEYWORD
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Boehmeria nivea, antioxidant activity, DPPH radical scavenging activity, nitrite scavenging ability, reducing power
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